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pssGFP-hCRT
(Plasmid #80022)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80022 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 80022-D50 50 μg of DNA in Tris buffer $495
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DNA 80022-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-hCRT
  • Backbone manufacturer
    Eggleton Lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRT
  • Alt name
    Calreticulin
  • Species
    H. sapiens (human)
  • Mutation
    signal peptide 17aa added before GFP-CRT, will target to ER
  • Entrez Gene
    CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Promoter CMV
  • Tags / Fusion Proteins
    • signal peptide (N terminal on backbone)
    • EGFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pssGFP-hCRT was a gift from Chinten James Lim (Addgene plasmid # 80022 ; http://n2t.net/addgene:80022 ; RRID:Addgene_80022)

  • For your References section:

    alpha-Integrin expression and function modulates presentation of cell surface calreticulin. Liu CC, Leclair P, Monajemi M, Sly LM, Reid GS, Lim CJ. Cell Death Dis. 2016 Jun 16;7:e2268. doi: 10.1038/cddis.2016.176. 10.1038/cddis.2016.176 PubMed 27310876
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