p8.74DNW
(Plasmid
#80390)
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PurposeNILV lentivirus packaging, integrase deficient. Non integrating lentivirus
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Depositing Labs
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCMVdR8.74
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Backbone manufacturerDull, et al. 1998, PMID 9765382
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3 point mutation in the integrase
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SpeciesSynthetic
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MutationD64V, N120L, W235E in pol
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p8.74DNW was a gift from Magnus Essand & Nicola Philpott (Addgene plasmid # 80390) -
For your References section:
Stable gene transfer to muscle using non-integrating lentiviral vectors. Apolonia L, Waddington SN, Fernandes C, Ward NJ, Bouma G, Blundell MP, Thrasher AJ, Collins MK, Philpott NJ. Mol Ther. 2007 Nov;15(11):1947-54. Epub 2007 Aug 14. 10.1038/sj.mt.6300281 PubMed 17700544