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pBItetDT960GFP
(Plasmid #80419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80419 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 80419-D50 50 μg of DNA in Tris buffer $495
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DNA 80419-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBiTet
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats
  • Species
    H. sapiens (human)
  • Entrez Gene
    DMPK (a.k.a. DM, DM1, DM1PK, DMK, MDPK, MT-PK)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene note: 886 / 960 CTG repeats confirmed using Nanopore sequencing, however there may be a variable number of CTG repeats between different preps due to the inherent instability of tandem repeats. Please screen multiple colonies to select the full length of repeats.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBItetDT960GFP was a gift from Thomas Cooper (Addgene plasmid # 80419 ; http://n2t.net/addgene:80419 ; RRID:Addgene_80419)

  • For your References section:

    RNase H-mediated degradation of toxic RNA in myotonic dystrophy type 1. Lee JE, Bennett CF, Cooper TA. Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4221-6. doi: 10.1073/pnas.1117019109. Epub 2012 Feb 27. 10.1073/pnas.1117019109 PubMed 22371589
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