pBItetDT12nGFP
(Plasmid #80420)

• Purpose: tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located
• Depositing Lab: Thomas Cooper
• Publication: Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4221-6. doi: 10.1073/pnas.1117019109. Epub 2012 Feb 27.

Backbone

• Vector backbone: pBiTet
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP and human DMPK exons 11-15 with 12 CTG repeats
• Species: H. sapiens (human)
• Entrez Gene: DMPK (a.k.a. DM, DM1, DM1PK, DMK, MDPK, MT-PK)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.