Purpose(Empty Backbone) Cloning vector compatible with ColE1 plasmids with medium copy number
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80773||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerLerner CG, Inouye M., Nucleic Acids Res. 1990;18:4631.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13/pUC Forward
- 3′ sequencing primer M13/pUC Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJPC13 was a gift from Gregory Phillips (Addgene plasmid # 80773 ; http://n2t.net/addgene:80773 ; RRID:Addgene_80773)
For your References section:New pSC101-derivative cloning vectors with elevated copy numbers. Peterson J, Phillips GJ. Plasmid. 2008 May;59(3):193-201. doi: 10.1016/j.plasmid.2008.01.004. Epub 2008 Mar 4. 10.1016/j.plasmid.2008.01.004 PubMed 18295880