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            Purpose(Empty Backbone) Cas9, mCherry, and sgRNA expression
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              Depositing Lab
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          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepCAS9
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              Vector typeMammalian Expression
 
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature30°C
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            Growth Strain(s)DH10B
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            Copy numberHigh Copy
 
Gene/Insert
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                Gene/Insert nameNone
 
Resource Information
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            Addgene Notes
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            Articles Citing this Plasmid
 
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAS9-mCherry empty was a gift from Veit Hornung (Addgene plasmid # 80975 ; http://n2t.net/addgene:80975 ; RRID:Addgene_80975) - 
                
For your References section:
CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Schmid-Burgk JL, Honing K, Ebert TS, Hornung V. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. 10.1038/ncomms12338 PubMed 27465542