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(Plasmid #80978)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 80978 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Steyger lab
  • Modifications to backbone
    GFP with the addition of KDEL was generated by PCR using pEGFP-N2 (Clontech) as a template, digested with NotI/XbaI and subcloned into pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    no KDEL
  • Entrez Gene
    Calr (a.k.a. CR, CRT, Calregulin)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRT-GFP pcDNA3.1 was a gift from Peter Steyger (Addgene plasmid # 80978 ; ; RRID:Addgene_80978)
  • For your References section:

    Calreticulin binds to gentamicin and reduces drug-induced ototoxicity. Karasawa T, Wang Q, David LL, Steyger PS. Toxicol Sci. 2011 Dec;124(2):378-87. doi: 10.1093/toxsci/kfr196. Epub 2011 Jul 23. 10.1093/toxsci/kfr196 PubMed 21785162