CRT-GFP pcDNA3.1
(Plasmid #80978)

• Purpose: mammalian expression of CRT-GFP
• Depositing Lab: Peter Steyger
• Publication: Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi: 10.1093/toxsci/kfr196. Epub 2011 Jul 23.

Backbone

• Vector backbone: GFP-pcDNA3.1
• Backbone manufacturer: Steyger lab
• Modifications to backbone: GFP with the addition of KDEL was generated by PCR using pEGFP-N2 (Clontech) as a template, digested with NotI/XbaI and subcloned into pcDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRT
• Alt name: calreticulin
• Species: M. musculus (mouse)
• Mutation: no KDEL
• Entrez Gene: Calr (a.k.a. CRT, Calregulin)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry