PurposeHO endonuclease expression
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||81043||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6123
Modifications to backbonepAG36 with Gal1/10 promoter added between CEN/ARS and HO added at EcoR1 site
Vector typeYeast Expression
Selectable markersNAT (select with Nourseothricin/clonNAT)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Promoter endogenous
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (unknown if destroyed)
- 3′ cloning site EcoR1 (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The plasmid contains HO with its endogenous promoter amplified from yCP50-HO with oligos :
Note on YCp50: HO sequence is as published in Meiron et al, Curr Genetics 1995, 367-373. Original source of plasmid may be from reference Jensen et al, 1983, Proc Natl Acad Sci USA 80: 3035-3039.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRY003 was a gift from John McCusker (Addgene plasmid # 81043 ; http://n2t.net/addgene:81043 ; RRID:Addgene_81043)