pVHed05
(Plasmid #82351)

• Purpose: Contains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR
• Depositing Lab: Richard Murray
• Publication: Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. doi: 10.15252/msb.20156663.

Backbone

• Vector backbone: ColE1
• Backbone size w/o insert (bp): 2200
• Total vector size (bp): 7352
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: TP901-1 integrase
• Insert Size (bp): 1527
• GenBank ID:

Gene/Insert 2

• Gene/Insert name: Bxb1 integrase
• Insert Size (bp): 1503

Gene/Insert 3

• Gene/Insert name: AraC
• Insert Size (bp): 878
• Entrez Gene: araC (a.k.a. b0064, ECK0065)

Gene/Insert 4

• Gene/Insert name: TetR
• Insert Size (bp): 624

Resource Information

• Supplemental Documents:
  • pVHed05 annotated plasmid map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is exactly the Dual-recombinase-controller (Addgene 44456) from Bonnet et al, with the addition of a constitutive wild type tetR gene.