Hs.NRAS Q61R
(Plasmid #83179)

• Purpose: Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
• Depositing Lab: Dominic Esposito
• Publication: RAS mutant collection V2.0 (unpublished)

Backbone

• Vector backbone: pDonor-255
• Backbone size w/o insert (bp): 2693
• Vector type: Gateway Entry Clone

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRAS
• Species: H. sapiens (human)
• Insert Size (bp): 573
• Mutation: Q61R
• GenBank ID: NM_002524.4
• Entrez Gene: NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
• 3′ sequencing primer: attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry