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p415 TEF roGFP2‐Tsa2ΔCPΔCR
(Plasmid #83239)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83239 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p415-TEF
  • Total vector size (bp) 8000
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    roGFP2-Tsa2dCpdCr
  • Species
    Synthetic
  • Insert Size (bp)
    1500
  • Promoter TEF
  • Tag / Fusion Protein
    • roGFP2 fusion with Tsa2dCpdCr (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p415 TEF roGFP2‐Tsa2ΔCPΔCR was a gift from Tobias Dick (Addgene plasmid # 83239 ; http://n2t.net/addgene:83239 ; RRID:Addgene_83239)
  • For your References section:

    Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes. Morgan B, Van Laer K, Owusu TN, Ezerina D, Pastor-Flores D, Amponsah PS, Tursch A, Dick TP. Nat Chem Biol. 2016 Jun;12(6):437-43. doi: 10.1038/nchembio.2067. Epub 2016 Apr 18. 10.1038/nchembio.2067 PubMed 27089028