p415 TEF Su9‐roGFP2‐Tsa2ΔCR
PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83240||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8000
Vector typeYeast Expression
Growth in Bacteria
- Promoter TEF
/ Fusion Protein
- roGFP2 fusion with Tsa2dCr with Su9 targetting sequence (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p415 TEF Su9‐roGFP2‐Tsa2ΔCR was a gift from Tobias Dick (Addgene plasmid # 83240 ; http://n2t.net/addgene:83240 ; RRID:Addgene_83240)
For your References section:Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes. Morgan B, Van Laer K, Owusu TN, Ezerina D, Pastor-Flores D, Amponsah PS, Tursch A, Dick TP. Nat Chem Biol. 2016 Jun;12(6):437-43. doi: 10.1038/nchembio.2067. Epub 2016 Apr 18. 10.1038/nchembio.2067 PubMed 27089028