pCFP.HXGPRT
(Plasmid
#83366)
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PurposePlasmid contains LIC cassette upstream of an in-frame copy of CFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus
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Depositing Lab
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Depositing OrganizationUniversity of Michigan
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 83366-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 83366-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepKS
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Vector typeToxoplasma gondii
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCFP
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Insert Size (bp)720
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Tag
/ Fusion Protein
- CFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 83366-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 83366-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFP.HXGPRT was a gift from Vernon Carruthers (Addgene plasmid # 83366 ; http://n2t.net/addgene:83366 ; RRID:Addgene_83366) -
For your References section:
Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80. Huynh MH, Carruthers VB. Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 2009 Feb 13. 10.1128/EC.00358-08 PubMed 19218426
Map uploaded by the depositor.
