GST-FOXO3a TM 1-506aa
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8351||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGex 4T3
- Backbone size w/o insert (bp) 4968
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFOXO3a 1-506aa
Alt nameforkhead family
SpeciesH. sapiens (human)
Insert Size (bp)1500
MutationT32A, S253A, S315A
Entrez GeneFOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Note that the stop codon in the vector backbone is used to terminate translation. Thus, a 10aa sequence (VDSSGRIVTD) will be expressed as part of FOXO3a.
See GST-FOXO3a 1-506aa for wild-type map and sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GST-FOXO3a TM 1-506aa was a gift from Michael Greenberg (Addgene plasmid # 8351)
For your References section:Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME. Cell 1999 Mar 19;96(6):857-68. 10.1016/S0092-8674(00)80595-4 PubMed 10102273