pGEM-rat FceRI alpha
(Plasmid
#8373)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEM 3Zf-
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3199
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Vector typeBacterial Expression ; Beta-gal
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerat Fc epsilon RI alpha cDNA
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Alt namehigh affinity receptor I for IgE alpha chain
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Alt nameFcer1a rat
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SpeciesR. norvegicus (rat)
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Insert Size (bp)811
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GenBank IDM17153
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Entrez GeneFcer1a (a.k.a. Iger01, RATIGER01)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-rat FceRI alpha was a gift from Jean Kinet (Addgene plasmid # 8373 ; http://n2t.net/addgene:8373 ; RRID:Addgene_8373) -
For your References section:
A cDNA presumptively coding for the alpha subunit of the receptor with high affinity for immunoglobulin E. Kinet JP, Metzger H, Hakimi J, Kochan J. Biochemistry 1987 Jul 28;26(15):4605-10. 10.1021/bi00389a002 PubMed 2959318