pGEM-rat Fcepsilon RI gamma
(Plasmid
#8375)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEM 3Zf-
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3199
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Vector typeBacterial Expression ; Beta-gal
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerat Fc epsilon RI gamma cDNA
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Alt nameFcerIg rat
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Alt nameFc receptor gamma chain rat
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Alt namehigh affinity receptor IgE rat
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SpeciesR. norvegicus (rat)
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Insert Size (bp)700
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GenBank IDL04306
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Entrez GeneFcer1g
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-rat Fcepsilon RI gamma was a gift from Jean Kinet (Addgene plasmid # 8375 ; http://n2t.net/addgene:8375 ; RRID:Addgene_8375) -
For your References section:
Complete structure and expression in transfected cells of high affinity IgE receptor. Blank U, Ra C, Miller L, White K, Metzger H, Kinet JP. Nature 1989 Jan 12;337(6203):187-9. 10.1038/337187a0 PubMed 2521376