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pGEM-rat FceRI beta cDNA
(Plasmid #8374)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8374 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM 4
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2871
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rat Fc epsilon RI beta chain
  • Alt name
    Fcer1b rat
  • Alt name
    high affinity receptor beta subunit rat
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1051
  • GenBank ID
    M22923
  • Entrez Gene
    Ms4a2 (a.k.a. FCIGA, Fcer1b, Ms4a1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-rat FceRI beta cDNA was a gift from Jean Kinet (Addgene plasmid # 8374 ; http://n2t.net/addgene:8374 ; RRID:Addgene_8374)
  • For your References section:

    Isolation and characterization of cDNAs coding for the beta subunit of the high-affinity receptor for immunoglobulin E. Kinet JP, Blank U, Ra C, White K, Metzger H, Kochan J. Proc Natl Acad Sci U S A 1988 Sep;85(17):6483-7. 10.1073/pnas.85.17.6483 PubMed 2970642