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pX601-GFP
(Plasmid #84040)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84040 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pX601
  • Backbone manufacturer
    Feng Zhang's Laboratory
  • Backbone size w/o insert (bp) 7447
  • Total vector size (bp) 8138
  • Modifications to backbone
    Insert GFP down stream of SaCas9
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • gRNA/shRNA sequence
    empty vector
  • Species
    Neisseria gonorrhoeae
  • GenBank ID
    7011691
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer Cas9-HindIII 5' -S:gccccgtcgtgaagagaagcttcatcc
  • 3′ sequencing primer EGFP-EcoR1-AS:cgtagaattcggccgctttacttgtacagctcgtccatgccga
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX601-GFP was a gift from Yuet Wai Kan (Addgene plasmid # 84040 ; http://n2t.net/addgene:84040 ; RRID:Addgene_84040)
  • For your References section:

    Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia. Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW. Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6. 10.1073/pnas.1612075113 PubMed 27601644