pMTH PKC alpha
(Plasmid
#8409)
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8409 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 8409-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 8409-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMTH
- Backbone size w/o insert (bp) 11500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse Protein Kinase C alpha
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Alt nameProtein Kinase C alpha
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Alt namePKC alpha
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SpeciesM. musculus (mouse)
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Insert Size (bp)2500
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GenBank IDM25811
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Entrez GenePrkca (a.k.a. Pkca)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe pMTH backbone was a gift from Dr. Michael Kuehl (backbone map uploaded here). PKC alpha was a gift from Dr. Stefan Rose-John.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 8409-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 8409-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMTH PKC alpha was a gift from Frederic Mushinski (Addgene plasmid # 8409 ; http://n2t.net/addgene:8409 ; RRID:Addgene_8409) -
For your References section:
Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta. Mischak H, Pierce JH, Goodnight J, Kazanietz MG, Blumberg PM, Mushinski JF. J Biol Chem 1993 Sep 25;268(27):20110-5. PubMed 8376369
Map uploaded by the depositor.
