pMK1221
(Plasmid
#84220)
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Purpose(Empty Backbone) Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherry
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84220 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSicoR
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK1221 was a gift from Martin Kampmann & Jonathan Weissman (Addgene plasmid # 84220 ; http://n2t.net/addgene:84220 ; RRID:Addgene_84220) -
For your References section:
Next-generation libraries for robust RNA interference-based genome-wide screens. Kampmann M, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS. Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3384-91. doi: 10.1073/pnas.1508821112. Epub 2015 Jun 15. 10.1073/pnas.1508821112 PubMed 26080438
Map uploaded by the depositor.
