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pTWIN1-His6-Ssp-Httex1-7Q
(Plasmid #84347)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84347 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTWIN1
  • Backbone manufacturer
    eBioLabs
  • Backbone size w/o insert (bp) 5800
  • Total vector size (bp) 6500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Huntingtin Exon 1
  • Alt name
    Httex1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal His6-tagged intein Ssp

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer TGCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The company GeneArt from Thermo Fisher Scientific performed the synthesis and subcloning
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTWIN1-His6-Ssp-Httex1-7Q was a gift from Hilal Lashuel (Addgene plasmid # 84347 ; http://n2t.net/addgene:84347 ; RRID:Addgene_84347)
  • For your References section:

    An Intein-based Strategy for the Production of Tag-free Huntingtin Exon 1 Proteins Enables New Insights into the Polyglutamine Dependence of Httex1 Aggregation and Fibril Formation. Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA. J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 10.1074/jbc.M116.713982. Epub 2016 Mar 21. 10.1074/jbc.M116.713982 PubMed 27002149