hNCAM2
(Plasmid #84473)

• Purpose: expression of hNCAM2 in mammalian cells
• Depositing Lab: Vladimir Sytnyk
• Publication: Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.1038/ncomms9836.

Backbone

• Vector backbone: pcDNA3.1TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5351
• Total vector size (bp): 8000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Growth instructions: Transformation/Growth instructions: The transformation procedure is as following: 1) Place the bacteria on ice for 5-10 min to thaw. 2) Add 50 ng of plasmid and incubate for 10 min on ice. 3) Water bath the bacteria at 42C for 30 sec. 4) Place the bacteria on ice for 2 min. 5) Add S.O.C. media. Incubate 2 hours at 20C. 6) Plate bacteria on the agar plate containing 350 ug/ml of ampicillin. 7) Grow bacteria for 48 hours at 20C. The resulting colonies should be small. 8) Pick the colonies up and grow them for 48 hours in LB medium supplemented with 350ug/ml of ampicillin at 20C.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NCAM2
• Species: H. sapiens (human)
• Insert Size (bp): 2541
• GenBank ID: NM_004540.3
• Entrez Gene: NCAM2 (a.k.a. NCAM21)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The gene was synthesized using GeneArt service of Life Technology

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry