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hNCAM2
(Plasmid #84473)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84473 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5351
  • Total vector size (bp) 8000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Transformation/Growth instructions: The transformation procedure is as following: 1) Place the bacteria on ice for 5-10 min to thaw. 2) Add 50 ng of plasmid and incubate for 10 min on ice. 3) Water bath the bacteria at 42C for 30 sec. 4) Place the bacteria on ice for 2 min. 5) Add S.O.C. media. Incubate 2 hours at 20C. 6) Plate bacteria on the agar plate containing 350 ug/ml of ampicillin. 7) Grow bacteria for 48 hours at 20C. The resulting colonies should be small. 8) Pick the colonies up and grow them for 48 hours in LB medium supplemented with 350ug/ml of ampicillin at 20C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NCAM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2541
  • GenBank ID
    NM_004540.3
  • Entrez Gene
    NCAM2 (a.k.a. NCAM21)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene was synthesized using GeneArt service of Life Technology

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hNCAM2 was a gift from Vladimir Sytnyk (Addgene plasmid # 84473 ; http://n2t.net/addgene:84473 ; RRID:Addgene_84473)
  • For your References section:

    Abeta-dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer's disease. Leshchyns'ka I, Liew HT, Shepherd C, Halliday GM, Stevens CH, Ke YD, Ittner LM, Sytnyk V. Nat Commun. 2015 Nov 27;6:8836. doi: 10.1038/ncomms9836. 10.1038/ncomms9836 PubMed 26611261