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pMycoFos
(Plasmid #84577)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84577 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Growth instructions
    Sample requires arabinose for induction.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For maintenance of vector, any standard E.coli is OK, but to make plasmid preps, the plasmid must be propagated in EPI300 or similar strain that enables the arabinose-inducible copy control system. Induction of gene expression is with acetamide in Mycobacterium, but this doesnt work in E.coli. Some level of control over expression in E.coli can be gained via controlling the copy number of the plasmid with arabinose. See published paper for more details on construction and use of pMycoFos. Has unique EcoRI, PacI, NheI, SwaI, BamHI sites for cloning

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMycoFos was a gift from Nicholas Coleman (Addgene plasmid # 84577 ; http://n2t.net/addgene:84577 ; RRID:Addgene_84577)
  • For your References section:

    Construction and evaluation of pMycoFos, a fosmid shuttle vector for Mycobacterium spp. with inducible gene expression and copy number control. Ly MA, Liew EF, Le NB, Coleman NV. J Microbiol Methods. 2011 Sep;86(3):320-6. doi: 10.1016/j.mimet.2011.06.005. Epub 2011 Jun 13. 10.1016/j.mimet.2011.06.005 PubMed 21689690