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pUS116
(Plasmid #84578)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84578 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Induction of gene expression is with acetamide in Mycobacterium, but this doesnt work in E.coli. This plasmid was made by deletion of the recombineering genes from pJV53 (see van Kessel and Hatfull, 2007), to re-form a basic shuttle/expression vector. pUS116 is equivalent to vector pLAM12. Has unique NdeI, BamHI, PvuII, EcoRI sites for cloning

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUS116 was a gift from Nicholas Coleman (Addgene plasmid # 84578 ; http://n2t.net/addgene:84578 ; RRID:Addgene_84578)