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(Plasmid #84578)


Item Catalog # Description Quantity Price (USD)
Plasmid 84578 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pUS116 is an E.coli/Mycobacterium shuttle vector, with high copy number in E.coli and low copy number in Mycobacterium. The plasmid has unique NdeI, BamHI, PvuII, EcoRI, NheI sites for cloning, and genes cloned at this MCS can be expressed via acetamide induction in Mycobacterium, but this system doesnt work in E.coli. This plasmid was made by deletion of the recombineering genes from pJV53 (see van Kessel and Hatfull, 2007), to re-forma basic shuttle/expression vector. pUS116 is equivalent to vector pLAM12.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUS116 was a gift from Nicholas Coleman (Addgene plasmid # 84578 ; ; RRID:Addgene_84578)