|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84578||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
* Login to view industry pricing.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
pUS116 is an E.coli/Mycobacterium shuttle vector, with high copy number in E.coli and low copy number in Mycobacterium. The plasmid has unique NdeI, BamHI, PvuII, EcoRI, NheI sites for cloning, and genes cloned at this MCS can be expressed via acetamide induction in Mycobacterium, but this system doesnt work in E.coli. This plasmid was made by deletion of the recombineering genes from pJV53 (see van Kessel and Hatfull, 2007), to re-forma basic shuttle/expression vector. pUS116 is equivalent to vector pLAM12.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUS116 was a gift from Nicholas Coleman (Addgene plasmid # 84578 ; http://n2t.net/addgene:84578 ; RRID:Addgene_84578)