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pMV306DIhsp+LuxG13
(Plasmid #49999)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49999 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMV306hsp
  • Backbone size w/o insert (bp) 6186
  • Total vector size (bp) 9507
  • Modifications to backbone
    Integrase gene removed by inverse PCR.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bacterial luciferase operon + G13 promoter
  • Alt name
    LuxABCDE
  • Species
    Mycobacterium marinum + Photorhabdus luminescens
  • Insert Size (bp)
    6186
  • Mutation
    It contains a gram-positive enhanced translation signal in front of luxA, luxC and luxe. G13 promoter cloned in front of luxC
  • Promoter G13 from M. marinum G13 promoter driving luxC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer agaataacgttggcactcgc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Genes were cloned from vector pSB2025 obtained from Dr. Phil Hill at Nottingham University.
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

The sequence of the Lux operon is theoretical. As such, there are a few differences between Addgene sequence and full plasmid sequence. These differences do not affect plasmid function.

Qazi, S.N., et al., agr expression precedes escape of internalized Staphylococcus aureus from the host endosome. Infect Immun, 2001. 69(11): p. 7074-82.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMV306DIhsp+LuxG13 was a gift from Brian Robertson (Addgene plasmid # 49999 ; http://n2t.net/addgene:49999 ; RRID:Addgene_49999)
  • For your References section:

    Rapid in vivo assessment of drug efficacy against Mycobacterium tuberculosis using an improved firefly luciferase. Andreu N, Zelmer A, Sampson SL, Ikeh M, Bancroft GJ, Schaible UE, Wiles S, Robertson BD. J Antimicrob Chemother. 2013 Sep;68(9):2118-27. doi: 10.1093/jac/dkt155. Epub 2013 Apr 30. 10.1093/jac/dkt155 PubMed 23633686