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Addgene

pUS232
(Plasmid #84579)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84579 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pUS232
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A derivative of pBBR1MCS-2. Non-mobilisable. Cloning site is compatible with iGEM cloning protocols (EcoRI-NotI-XbaI-SpeI-NotI-PstI) and Golden-Gate cloning protocols (BsaI-BsaI). Blue/white selection is via excision of lacZalpha fragment, rather than interruption of this. Has unique ScaI, BamHI, SmaI, EcoRV, EcoRI, XbaI, StuI, SpeI, PstI, HindIII, ClaI, SalI sites for cloning. Has two NotI and BsaI sites for cloning

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUS232 was a gift from Nicholas Coleman (Addgene plasmid # 84579 ; http://n2t.net/addgene:84579 ; RRID:Addgene_84579)