|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17806||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5135
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The PCR product containing prpR and PprpB was digested with ClaI and NheI and ligated to the large fragments of pBAD18 resulting from digestion with the same enzymes, creating pPro18.
Please refer to the Addgene verified sequence for the correct orientation of the MCS.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPro18 was a gift from Jay Keasling (Addgene plasmid # 17806 ; http://n2t.net/addgene:17806 ; RRID:Addgene_17806)
For your References section:A propionate-inducible expression system for enteric bacteria. Lee SK, Keasling JD. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62. 10.1128/AEM.71.11.6856-6862.2005 PubMed 16269719