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pPro30
(Plasmid #17809)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17809 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPro30
  • Backbone size (bp) 5445
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

After deletion of the NheI site in the front of pACYC origin region of pBAD30 by partial digestion with NheI, T4 DNA polymerase treatment, and self-ligation, the ClaI-NheI prpR-PprpB region from pPro18 was ligated to the large fragments of pBAD30 resulting from digestion with the same enzymes, creating pPro30.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPro30 was a gift from Jay Keasling (Addgene plasmid # 17809 ; http://n2t.net/addgene:17809 ; RRID:Addgene_17809)
  • For your References section:

    A propionate-inducible expression system for enteric bacteria. Lee SK, Keasling JD. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62. 10.1128/AEM.71.11.6856-6862.2005 PubMed 16269719