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pCis GFPuv-SS Himar1 A7
(Plasmid #84686)


Item Catalog # Description Quantity Price (USD)
Plasmid 84686 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Vector type
    Bacterial Expression
  • Selectable markers
    Spectinomycin and Streptomycin, GFPuv

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Depositor Comments

Based on pMarGent, received from the RML ( The flgB promoter was replaced with an optimized Anaplasma marginale tr promoter. Initially, we used a two-plasmid system with the transposase and transposon encoded on two separate plasmids, described here: . pCis GFPuv-SS Himar1 A7 contains transposase and transposon in one plasmid and is described in Chapter 14 of Intracellular Pathogens II: Rickettsiales, Edited by G. H. Palmer and A. F. Azad © 2012 ASM Press, Washington, DC, 10.1128/9781555817336.ch14

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCis GFPuv-SS Himar1 A7 was a gift from Ulrike Munderloh (Addgene plasmid # 84686 ; ; RRID:Addgene_84686)
  • For your References section:

    The Way Forward: Improving Genetic Systems. Munderloh UG, Felsheim RF, Burkhardt NY, Herron MJ, Oliva Chavez AS, Nelson CM, Kurtti TJ. Intracellular Pathogens II: Rickettsiales. Vol. 89, No. 3, 2012. Chapter 14. eds: Palmer GH, and Azad AF. ASM Press, New York. 10.1086/677609