Cathepsin S S21C/C25A
(Plasmid #84695)

• Purpose: for expression of mutant Cathepsin S in Leishmania tarantolae
• Depositing Lab: Hans Brandstetter
• Publication: Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.1038/srep12707.

Backbone

• Vector backbone: pAPSPEGFP1.4sat
• Backbone manufacturer: Jena Bioscience
• Backbone size w/o insert (bp): 7655
• Vector type: expression in Leishmania tarantolae
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cathepsin S
• Alt name: antigenprocessing proteases
• Species: H. sapiens (human)
• Insert Size (bp): 945
• Mutation: S21C, C25A
• Promoter: unknown
• Tag / Fusion Protein:
  • his-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

expression constructs contain an N-terminal signal sequence for secretory expression, followed by an N-terminal His6-tag for purification, which remains with the propeptide after autoactivation