|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8484||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4245
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Alt nametnsB tnsC
Alt nametnsD tnsE
Insert Size (bp)8552
Entrez GenetnsA (a.k.a. R721_29)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer na (Common Sequencing Primers)
pCW4 sequence is not complete: the entire plasmid is not known (provided sequence is simply a mosaic of the known pieces). Note: Sequence around 8554 RI site uncertain, there is about 800 bp extra between the first RI site and the adjacent HIII site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCW4 was a gift from Nancy Craig (Addgene plasmid # 8484 ; http://n2t.net/addgene:8484 ; RRID:Addgene_8484)
For your References section:Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes. Waddell CS, Craig NL. Genes Dev 1988 Feb;2(2):137-49. 10.1101/gad.2.2.137 PubMed 2834269