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(Plasmid #8484)


Item Catalog # Description Quantity Price (USD)
Plasmid 8484 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4245
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    tnsB tnsC
  • Alt name
    tnsD tnsE
  • Species
  • Insert Size (bp)
  • Entrez Gene
    tnsA (a.k.a. R721_29)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCW4 sequence is not complete: the entire plasmid is not known (provided sequence is simply a mosaic of the known pieces). Note: Sequence around 8554 RI site uncertain, there is about 800 bp extra between the first RI site and the adjacent HIII site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW4 was a gift from Nancy Craig (Addgene plasmid # 8484 ; ; RRID:Addgene_8484)
  • For your References section:

    Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes. Waddell CS, Craig NL. Genes Dev 1988 Feb;2(2):137-49. 10.1101/gad.2.2.137 PubMed 2834269