pCW15
(Plasmid
#8485)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepACYC184
-
Backbone manufacturerNEB
- Backbone size w/o insert (bp) 4244
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameTn7ABC
-
Alt nametnsA
-
Alt nametnsB
-
Alt nametnsC
-
SpeciesBacteria
-
Insert Size (bp)4923
-
Entrez GenetnsA (a.k.a. R721_29)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (destroyed during cloning)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCW15 was a gift from Nancy Craig (Addgene plasmid # 8485 ; http://n2t.net/addgene:8485 ; RRID:Addgene_8485) -
For your References section:
Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes. Waddell CS, Craig NL. Genes Dev 1988 Feb;2(2):137-49. 10.1101/gad.2.2.137 PubMed 2834269
