pAG303-Gal-GA50
(Plasmid
#84907)
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Purposeinduce expression of dipeptide repeat GA in yeast
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAG303-Gal-ccdB
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Backbone manufacturerSusan Lindquist (Addgene plasmid # 14133)
- Backbone size w/o insert (bp) 5192
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGA50
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Alt nameC9orf72 dipeptide
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SpeciesSynthetic
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Insert Size (bp)378
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Entrez GeneC9orf72 (a.k.a. ALSFTD, DENND9, DENNL72, FTDALS, FTDALS1)
- Promoter GAL1
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Tags
/ Fusion Proteins
- Myc (C terminal on insert)
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG303-Gal-GA50 was a gift from Aaron Gitler (Addgene plasmid # 84907 ; http://n2t.net/addgene:84907 ; RRID:Addgene_84907) -
For your References section:
Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS. Jovicic A, Mertens J, Boeynaems S, Bogaert E, Chai N, Yamada SB, Paul JW 3rd, Sun S, Herdy JR, Bieri G, Kramer NJ, Gage FH, Van Den Bosch L, Robberecht W, Gitler AD. Nat Neurosci. 2015 Sep;18(9):1226-9. doi: 10.1038/nn.4085. 10.1038/nn.4085 PubMed 26308983
