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UbcH10 AU1
(Plasmid #8506)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8506 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 8506-D50 50 μg of DNA in Tris buffer $495
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DNA 8506-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pJS55
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcH10
  • Alt name
    ubiquitin-conjugating enzyme H10
  • Alt name
    E2-C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    561
  • Entrez Gene
    UBE2C (a.k.a. UBCH10, dJ447F3.2)
  • Tag / Fusion Protein
    • AU1 (DTYRYI) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. Expression of tagged UbcH10 protein in mammalian cells from early SV40 promoter. Antibodies to AU1 epitope available from BABCO. (Ruderman pJHEAUC+2)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbcH10 AU1 was a gift from Joan Ruderman (Addgene plasmid # 8506 ; http://n2t.net/addgene:8506 ; RRID:Addgene_8506)

  • For your References section:

    Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase. Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV. Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2362-7. 10.1073/pnas.94.6.2362 PubMed 9122200
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