pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
(Plasmid #85111)

• Purpose: expression of recombinant human mature (delta19) ETFalpha wt without tag
• Depositing Lab: Pal Falnes
• Publication: Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115. Epub 2014 Nov 21.

Backbone

• Vector backbone: pETDuet-1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: human mature (delta19) ETFalpha
• Species: H. sapiens (human)
• Insert Size (bp): 945
• Entrez Gene: ETFA (a.k.a. EMA, GA2, MADD)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TTGTACACGGCCGCATAATC
• 3′ sequencing primer: TAGAGGCCCCAAGGGGTTATGCTAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Homo sapiens wt ETF alpha, delta 19, cloned into NdeI-XhoI sites of pETDuet-1, without tag