pYES260-sc.eEF2
(Plasmid #85119)

• Purpose: yeast expression of EEF2
• Depositing Lab: Pal Falnes
• Publication: Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-510. doi: 10.1074/jbc.M114.601658. Epub 2014 Sep 17.

Backbone

• Vector backbone: pYES260
• Backbone manufacturer: Euroscarf (Melcher)
• Backbone size w/o insert (bp): 5933
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EFT1/EFT2
• Alt name: EEF2
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2529
• Entrez Gene: EFT2 (a.k.a. YDR385W)
• Entrez Gene: EFT1 (a.k.a. YOR133W)
• Promoter: GAL1
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GAL1
• 3′ sequencing primer: CYC1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EFT1 and EFT2 are paralogs that arose through duplication. They have identical amino acid sequence.