Purposeyeast expression of EEF2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85119||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerEuroscarf (Melcher)
- Backbone size w/o insert (bp) 5933
Vector typeYeast Expression
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GAL1
- 3′ sequencing primer CYC1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
EFT1 and EFT2 are paralogs that arose through duplication. They have identical amino acid sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYES260-sc.eEF2 was a gift from Pal Falnes (Addgene plasmid # 85119 ; http://n2t.net/addgene:85119 ; RRID:Addgene_85119)
For your References section:Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2). Davydova E, Ho AY, Malecki J, Moen A, Enserink JM, Jakobsson ME, Loenarz C, Falnes PO. J Biol Chem. 2014 Oct 31;289(44):30499-510. doi: 10.1074/jbc.M114.601658. Epub 2014 Sep 17. 10.1074/jbc.M114.601658 PubMed 25231979