pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)
(Plasmid #85179)

• Purpose: For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.
• Depositing Lab: Takuji Iwasato
• Publication: Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.

Backbone

• Vector backbone: pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
• Backbone size w/o insert (bp): 9530
• Total vector size (bp): 10280
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nuclear localization signal (nls) tagRFP
• Species: SV40, Entacmaea quadricolor
• Insert Size (bp): 750

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: EBV_rev_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone plasmid (pK038) is available in Addgene.