Purposemammalian expression of ubiquitin-K48R-EGFP fusion
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85219||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameubiquitin C
SpeciesH. sapiens (human)
Entrez GeneUBC (a.k.a. HMG20)
- Promoter human ubiquitin C
/ Fusion Proteins
- 6xHIS (N terminal on insert)
- EGFP (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer hUBCpro-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDG279 was a gift from Douglas Gray (Addgene plasmid # 85219 ; http://n2t.net/addgene:85219 ; RRID:Addgene_85219)
For your References section:Sensitivity of mammalian cells expressing mutant ubiquitin to protein-damaging agents. Tsirigotis M, Zhang M, Chiu RK, Wouters BG, Gray DA. J Biol Chem. 2001 Dec 7;276(49):46073-8. 10.1074/jbc.M109023200 PubMed 11598140