Skip to main content
Addgene
Open Menu

PB-Dox-hCas9-m4-KRAB-EBFP2
(Plasmid #85340)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85340 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    NAX-TRE
  • Total vector size (bp) 12029
  • Vector type
    Mammalian Expression ; Piggybac
  • Selectable markers
    EBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-m4-KRAB
  • Species
    Synthetic
  • Insert Size (bp)
    4392
  • Promoter TRE
  • Tag / Fusion Protein
    • KRAB (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer LNCX (AGCTCGTTTAGTGAACCGTCAGATC)
  • 3′ sequencing primer TK-pA-R (5'ttgtctccttccgtgtttca)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-Dox-hCas9-m4-KRAB-EBFP2 was a gift from George Church (Addgene plasmid # 85340)
Related items: