p-mCherry-N1-iChloC
(Plasmid #85467)

• Purpose: improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)
• Depositing Lab: Peter Hegemann
• Publication: Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.1038/srep14807.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 5660
• Modifications to backbone: EGFP has been replaced by inserting the complete fusion construct (ChR2-mCherry) using XbaI and BamHI cloning sites
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iChloC
• Alt name: Channelrhodopsin-2
• Alt name: ChR2
• Species: Synthetic; Chlamydomonas reinhardtii
• Insert Size (bp): 927
• Mutation: E83Q, E90R, E101S, T159C, D156N
• GenBank ID: EU714030.1
• Promoter: CMV (+enhancer)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-N1-F: GAGGTCTATATAAGCAGAGC or CMV-F: GCAAATGGGCGGTAGGCGT
• 3′ sequencing primer: EGFP-C1-R: AACCATTATAAGCTGCAATAAAC

Resource Information

• Supplemental Documents:
  • 199_iChloC_mCherry.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry