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pGST-PSP-hTG
(Plasmid #85470)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85470 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX
  • Modifications to backbone
    a PreScission Protease site (PSP) was introduced by a site-overlap extension mutation strategy
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hTG2
  • Alt name
    transglutaminase 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    TGM2 (a.k.a. TG(C), TGC)
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • PSP (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST-PSP-hTG was a gift from Jeffrey Keillor & Ray Truant (Addgene plasmid # 85470 ; http://n2t.net/addgene:85470 ; RRID:Addgene_85470)
  • For your References section:

    Expression, purification and kinetic characterisation of human tissue transglutaminase. Roy I, Smith O, Clouthier CM, Keillor JW. Protein Expr Purif. 2013 Jan;87(1):41-6. doi: 10.1016/j.pep.2012.10.002. Epub 2012 Oct 22. 10.1016/j.pep.2012.10.002 PubMed 23085087