pMSCV-SMYD2 F184A
(Plasmid
#85659)
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Purposeretroviral expression of human SMYD2 F184A
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMSCV
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSMYD2
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SpeciesH. sapiens (human)
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MutationF184A
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Entrez GeneSMYD2 (a.k.a. HSKM-B, KMT3C, ZMYND14)
- Promoter LTR
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Tag
/ Fusion Protein
- Flag
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-SMYD2 F184A was a gift from Julien Sage (Addgene plasmid # 85659 ; http://n2t.net/addgene:85659 ; RRID:Addgene_85659) -
For your References section:
Coordination of stress signals by the lysine methyltransferase SMYD2 promotes pancreatic cancer. Reynoird N, Mazur PK, Stellfeld T, Flores NM, Lofgren SM, Carlson SM, Brambilla E, Hainaut P, Kaznowska EB, Arrowsmith CH, Khatri P, Stresemann C, Gozani O, Sage J. Genes Dev. 2016 Apr 1;30(7):772-85. doi: 10.1101/gad.275529.115. Epub 2016 Mar 17. 10.1101/gad.275529.115 PubMed 26988419
