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pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
(Plasmid #85821)


Item Catalog # Description Quantity Price (USD)
Plasmid 85821 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Chi-Bin Chen
  • Backbone size w/o insert (bp) 6141
  • Total vector size (bp) 11582
  • Vector type
    Tol2 based vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Zf Ubi::cMyc oDam_f_GFPoNLS
  • Alt name
    Ubi::cMyc oDamGFP
  • Species
    Ubi (Ubiquitin-B promoter): Danio rerio (Zebrafish); Dam: E. coli;. GFP: Aequorea victoria
  • Insert Size (bp)
  • Mutation
    L122A. Codon optimization compatible to work in Medaka and Zebrafish (other species not tested). Cryptic splicing sites removed.
  • Tags / Fusion Proteins
    • cMyc N terminal to oDam_f_GFPoNLS
    • oNLS (optimized nuclear localization signal) C terminal to the GFP

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS was a gift from Joachim Wittbrodt (Addgene plasmid # 85821 ; ; RRID:Addgene_85821)
  • For your References section:

    iDamIDseq and iDEAR: An improved method and computational pipeline to profile chromatin-binding proteins. Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J. Development. 2016 Oct 5. pii: dev.139261. 10.1242/dev.139261 PubMed 27707796