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(Plasmid #85823)


Item Catalog # Description Quantity Price (USD)
Plasmid 85823 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 7050
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Heme oxygenase 1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    HMOX1 (a.k.a. HMOX1D, HO-1, HSP32, bK286B10)
  • Promoter CMV-F
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • APEX2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3_HMOX1-V5-APEX2 was a gift from Hyun-Woo Rhee (Addgene plasmid # 85823 ; ; RRID:Addgene_85823)
  • For your References section:

    APEX Fingerprinting Reveals the Subcellular Localization of Proteins of Interest. Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW. Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 10.1016/j.celrep.2016.04.064. 10.1016/j.celrep.2016.04.064 PubMed 27184847