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pCDNA3_V5-APEX2-HMOX1
(Plasmid #85824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85824 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 85824-D50 50 μg of DNA in Tris buffer $495
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DNA 85824-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 7053
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V5-APEX2-HMOX1
  • Alt name
    Heme oxygenase 1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1671
  • GenBank ID
    NM_002133.2.
  • Entrez Gene
    HMOX1 (a.k.a. HMOX1D, HO-1, HSP32, bK286B10)
  • Promoter CMV-F
  • Tags / Fusion Proteins
    • V5 (N terminal on insert)
    • APEX2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3_V5-APEX2-HMOX1 was a gift from Hyun-Woo Rhee (Addgene plasmid # 85824 ; http://n2t.net/addgene:85824 ; RRID:Addgene_85824)

  • For your References section:

    APEX Fingerprinting Reveals the Subcellular Localization of Proteins of Interest. Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW. Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 10.1016/j.celrep.2016.04.064. 10.1016/j.celrep.2016.04.064 PubMed 27184847
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