pOP512
(Plasmid
#85940)
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PurposeBi-stable switch. From Collins lab but the origin substituted for a P15A origin.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBi-stable switch
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOP512 was a gift from Timothy Lu (Addgene plasmid # 85940 ; http://n2t.net/addgene:85940 ; RRID:Addgene_85940) -
For your References section:
Encryption and steganography of synthetic gene circuits. Purcell O, Wang J, Siuti P, Lu TK. Nat Commun. 2018 Nov 22;9(1):4942. doi: 10.1038/s41467-018-07144-7. 10.1038/s41467-018-07144-7 PubMed 30467337