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(Plasmid #86015)


Item Catalog # Description Quantity Price (USD)
Plasmid 86015 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pQLinkN (Addgene #13670)
  • Backbone manufacturer
    Konrad Buessow lab
  • Vector type
    Bacterial Expression ; Co-Expression; Purification

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Entrez Gene
    SNORD14A (a.k.a. RNU14, RNU14A, U14, U14-S13-5)

Gene/Insert 2

  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Entrez Gene
    SNU13 (a.k.a. 15.5K, FA-1, FA1, NHP2L1, NHPX, OTK27, SNRNP15-5, SPAG12, SSFA1)

Gene/Insert 3

  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    amino acids 83-316
  • Entrez Gene
    FBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)

Gene/Insert 4

  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    amino acids 1-411
  • Entrez Gene
    NOP56 (a.k.a. NOL5A, SCA36)
  • Tag / Fusion Protein
    • 6x-His (C terminal on insert)

Gene/Insert 5

  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    amino acids 1-401
  • Entrez Gene
    NOP58 (a.k.a. HSPC120, NOP5, NOP5/NOP58)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see associated publication for additional graphics describing this plasmid.

Plasmid construction: To construct the human box C/D plasmid, pQhsCD, pQLinkN (Addgene #13670) was inserted with the coding sequences of Homo sapiens (Hs) NOP56 (amino acids 1 to 411), NOP58 (amino acids 1 to 401) and fibrillarin (amino acids 83 to 316), 15.5 K, and U14 snoRNA. This plasmid differs from #86014 in that U14 is not part of a tRNA-containing minigene.

Each protein-encoding gene was cloned into pQLink-N plasmid using BamHI and NotI sites. The plasmids containing two different protein-encoding genes were then digested by SwaI and PacI, respectively, treated with LIC qualified T4 polymerase (dCTP for Pac I digest and dGTP for Swa I digest) and then annealed at 70°C. Each clone was identified by digestion of the recombined plasmid with Pac I. This process was repeated for additional coding sequence until all were inserted.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQhsCDtRNA- was a gift from Hong Li (Addgene plasmid # 86015 ; ; RRID:Addgene_86015)
  • For your References section:

    Co-expression and co-purification of archaeal and eukaryal box C/D RNPs. Peng Y, Yu G, Tian S, Li H. PLoS One. 2014 Jul 31;9(7):e103096. doi: 10.1371/journal.pone.0103096. eCollection 2014. PONE-D-14-15013 [pii] PubMed 25078083