Occludin_Flag
(Plasmid
#86042)
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PurposeFull length human occludin
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86042 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFlag CMV-6C
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Backbone manufacturerSigmaAldrich
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOccludin
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SpeciesH. sapiens (human)
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Insert Size (bp)1601
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MutationI52T and Q365P
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GenBank IDNM_002538.3
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Entrez GeneOCLN (a.k.a. BLCPMG, PPP1R115, PTORCH1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer C-CMV-24 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Occludin_Flag was a gift from Christie Thomas (Addgene plasmid # 86042 ; http://n2t.net/addgene:86042 ; RRID:Addgene_86042) -
For your References section:
Nedd4-2 interacts with occludin to inhibit tight junction formation and enhance paracellular conductance in collecting duct epithelia. Raikwar NS, Vandewalle A, Thomas CP. Am J Physiol Renal Physiol. 2010 Aug;299(2):F436-44. doi: 10.1152/ajprenal.00674.2009. Epub 2010 May 26. 10.1152/ajprenal.00674.2009 PubMed 20504882
