Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8608||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2629
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)439
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Barstar production plasmid. Barstar gene from Bacillus amyloliquefaciens strain H. 7-110: tac promoter and RBS.
111-445: DNA from B. amyloliquefaciens.
131-403: barstar structural gene Genbank #X15545. Grown in Super Broth at 37^0 C, yield, reaching o.5 to 1 g/l, increases for 10-12 hours after growth stops. Barstar is intracellular.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMT316 was a gift from Robert Hartley (Addgene plasmid # 8608 ; http://n2t.net/addgene:8608 ; RRID:Addgene_8608)
For your References section:Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. Hartley RW. J Mol Biol 1988 Aug 20;202(4):913-5. 10.1016/0022-2836(88)90568-2 PubMed 3050134