Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #8607)


Item Catalog # Description Quantity Price (USD)
Plasmid 8607 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Requires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE and HB107)
  • Copy number
    High Copy


  • Gene/Insert name
    barnase, barstar
  • Species
    Bacillus amyloliquefaciens
  • Insert Size (bp)
  • GenBank ID
    M14442 X15545
  • Tag / Fusion Protein
    • phoA signal sequence to barnase (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Barnase production plasmid. Same as pMT413 except vector is a reversed subsequence of pUC. Requires an E. coli host carrying the lacI^Q gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT416 was a gift from Robert Hartley (Addgene plasmid # 8607 ; ; RRID:Addgene_8607)
  • For your References section:

    Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. Hartley RW. J Mol Biol 1988 Aug 20;202(4):913-5. 10.1016/0022-2836(88)90568-2 PubMed 3050134