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pMT416
(Plasmid #8607)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8607 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Requires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE and HB107)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    barnase, barstar
  • Species
    Bacillus amyloliquefaciens
  • Insert Size (bp)
    1083
  • GenBank ID
    M14442 X15545
  • Tag / Fusion Protein
    • phoA signal sequence to barnase (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Barnase production plasmid. Same as pMT413 except vector is a reversed subsequence of pUC. Requires an E. coli host carrying the lacI^Q gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT416 was a gift from Robert Hartley (Addgene plasmid # 8607 ; http://n2t.net/addgene:8607 ; RRID:Addgene_8607)
  • For your References section:

    Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. Hartley RW. J Mol Biol 1988 Aug 20;202(4):913-5. 10.1016/0022-2836(88)90568-2 PubMed 3050134