|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8607||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepartial pUC19
- Backbone size w/o insert (bp) 1817
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsRequires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE and HB107)
Copy numberHigh Copy
Gene/Insert namebarnase, barstar
Insert Size (bp)1083
GenBank IDM14442 X15545
/ Fusion Protein
- phoA signal sequence to barnase (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Barnase production plasmid. Same as pMT413 except vector is a reversed subsequence of pUC. Requires an E. coli host carrying the lacI^Q gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMT416 was a gift from Robert Hartley (Addgene plasmid # 8607 ; http://n2t.net/addgene:8607 ; RRID:Addgene_8607)
For your References section:Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. Hartley RW. J Mol Biol 1988 Aug 20;202(4):913-5. 10.1016/0022-2836(88)90568-2 PubMed 3050134